hyperspectral_microscopy_image_analysis_using_imagej
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| hyperspectral_microscopy_image_analysis_using_imagej [2014/08/12 15:47] – stephsam | hyperspectral_microscopy_image_analysis_using_imagej [2022/08/26 12:10] (current) – old revision restored (2014/08/12 15:48) cosine | ||
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| Once you have loaded your image stacks for the sample and substrate as well as the single frame of dark current you will perform calculations as follows: | Once you have loaded your image stacks for the sample and substrate as well as the single frame of dark current you will perform calculations as follows: | ||
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| 1. Measure the mean value of the dark current image (Analyze> | 1. Measure the mean value of the dark current image (Analyze> | ||
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| 2. Subtract the dark current mean value from the sample and substrate stacks (Process> | 2. Subtract the dark current mean value from the sample and substrate stacks (Process> | ||
| - | 3. Perform image calculation to subtract the substrate image from the sample image (Process> | + | |
| + | 3. Perform image calculation to subtract the substrate image from the sample image (Process> | ||
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| 4. Perform another image calculation to divide the result of step 3 by the substrate image. | 4. Perform another image calculation to divide the result of step 3 by the substrate image. | ||
hyperspectral_microscopy_image_analysis_using_imagej.1407883678.txt.gz · Last modified: 2014/08/12 15:47 by stephsam